Tocotrienol Mitigating Adverse Effect of Doxorubicin on Pancreas Tissue in Male Rats

Hazar Shakir Saleh, Hayder Nadhim Ali

Abstract


Doxorubicin (DAX) as chemotherapy agent, it is known to induced acute pancreatitis in a patient with breast cancer, Tocotrienol (T3) has multiple biological properties such as antioxidant and anti-inflammatory agent. The role of T3 in pancreas protection during chemotherapy still not investigated. Aim: study has undertaken to investigate the role of T3 in the preservation of DAX-damaged pancreas. Method:  thirty male rats were divided into five treatment groups, namely, normal saline, olive oil only, T3 only, DAX and DAX with T3. The treatment was given for 30 days. After killing, pancreas tissues were eviscerated and examined histologically. Results: pancreas tissues were significant reduction in size in the DAX group compared with the normal group (DAX versus normal, mean area ±SD; 3.12±0.24 vs. 5.61±0.28 cm2; p<0.005), whilst synchronized administration of T3 with DAX leads to conservation of pancreas tissues (DAX+ T3 vs. DAX, mean area ± SD; 3.12±0.24 vs. 4.35 ± 0.18 cm2; p<   0.005). DAX showed abnormal atrophy in islet of Langerhans with highly degeneration in beta cells, interstitial edema, increased vascularity and inflammatory cell infiltration. These changes were reversed by concurrent T3 administration with DAX. To our knowledge, this is the first case of protection acute pancreatitis as deleterious effects of DAX administration by T3 supplementation.

Keywords: Tocotrienol, pancreas tissues, antioxidant, DAX.


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