Expression of The Vacuolar Aspartic Proteinase (APR1) Gene in Candida Albicans

Abstract

Intracellular proteinase A is an aspartic enzyme in yeast Candida albicans that is expressed by APR1 gene. APR1 Gene expression was evaluated in Candida albicans strains isolated from two Iranian patients, mice blood and one C. albicans ATCC strain. In this study 18S rRNA was considered as housekeeping gene. Reverse Transcriptase Polymerase Chain Reaction (RT-PCR) was applied to evaluate the APR1 gene and 18S rRNA using 2 pairs primers. The expression of APR1 and 18S rRNA genes was determined on SDS/polyacrylamide-gel electrophoresis. The expected size of the amplicon was about 220 bp for the 18SrRNA gene and 302 bp for the APR1 gene. APR1 gene expression in C. albicans isolated from mice blood was significantly higher than human patients (p<0.05).