Molecular detection of β-lactamase (tem and shv) genes in Escherichia coli O157:H7 isolated from different sources in Basra, Iraq

Basil A. Abbas, Bassam Y. Khudaier, Khulood A. Khaleel

Abstract


A total of 91 non-sorbitol fermenting Escherichia coli previously isolated from stool of hospitalized children suffering from diarrhea, beef samples and raw milk samples were used in this study. Among these, 29 strains were found enterohemorrhagic E. coli (EHEC) and only four strains were identified as Escherichia coli O157:H7 depending on latex agglutination test. Escherichia coli O157:H7 were further examined by amplifying both tem and shv genes. The results revealed that all isolates were positive to tem gen, 50% of them carrying shv gene and 50% of isolates carrying both tem and shv genes.


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