Pathological Changes of Dibenzo[a,l]Pyrene-Induced Peripheral T-Cell Non -Hodgkin’s -Lymphoma in Balb/C Mice

Abstract

Dibenzo[a,l]pyrene (DB[a,l]P) is one of the polycyclic aromatic hydrocarbons (PAHs) family which are present in the atmosphere from combustion sources such as diesel exhaust, residential heating processes, cigarette smoke and industrial coke production. The carcinogenic effects of Dibenzo[a,l]-pyrene (DBP) in inducing lymphoma in BALB/c mice is not studied yet. The gross pathology, histopathology and immunohistochemical alterations induced by DBP in BALB/c mice were determined. Forty adult female BALB/c mice were divided equally into two groups, the first one gavaged orally using DB[a,l]P (SIGMA-ALDRICHV) dissolved in corn oil ( SIGMA-ALDRICHV) at 15mg/kg of body weight while the second group was gavaged by corn oil only as a control group. Animals sacrificed after six months using ketamin: xylazin anesthesia. All animals’ organs were examined grossly for any changes in color, size, weight and the presence of any abnormal lesions. Statically significant difference appeared in lymph nodes, spleens, livers and kidneys weight compared to the control group due to enlargement of these organs in lymphoma group. Histopathological examinations showed that lymphoma induced in almost all mice organs including lymph nodes, thymus, spleen, liver, kidneys, heart, stomach, skin, small and large intestine and the brain. Immunohistochemical examinations showed that lymphoma induced by DBP in all mice organs is of T type non-Hodgkin’s lymphoma.